Ionization of Zwitterionic Amine Substrates Bound to Monomeric Sarcosine Oxidase
نویسندگان
چکیده
منابع مشابه
Novel substrates and products of amine oxidase-catalysed reactions.
Introduction Mitochondria1 monoamine oxidase (MAO; EC 1.4.3.4) catalyses the oxidative deamination of a variety of primary, secondary and tertiary amines. Until very recently most of the attention concerning MA0 activity was focused on the reaction of this enzyme to deaminate neurotransmitter substances, including adrenaline, noradrenaline, serotonin and dopamine. The ability of MA0 to regulate...
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The activity of highly purified bovine aortic lysyl oxidase against an insoluble aortic elastin substrate is inhibited by lysine, nonpeptidyl derivatives of lysine, P-aminopropionitrile (BAPN), and aliphatic monoand diamines. Inhibition requires the presence of a primary amino function. Although other stereochemical requirements for inhibition are not unusually rigid, inhibition is sensitive to...
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Monomeric sarcosine oxidase (MSOX) is a flavoprotein D-amino acid oxidase with reported sarcosine and oxygen activation sites on the re and si faces of the flavin ring, respectively. O2 transport routes to the catalytic interior are not well understood and are difficult to ascertain solely from MSOX crystal structures. A composite free-energy method known as single-sweep is used to map and ther...
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Introduction The concentration of serum creatinine is widely used as an indicator of renal function. Sarcosine oxidase (SO) has been used in the enzymatic determination of creatinine in the clinical laboratory. SO has been isolated from various bacterial strains. It was first purified from Corynebacterium sp. U-96 (SO-U96) and shown to be a heterotetrameric enzyme. SO-U96 is a complex, bifuncti...
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It has been established that the enzyme, sarcosine oxidase, found in the mitochondria of rat liver and elsewhere (I), catalyzes the following over-all reaction (2, 3) : sarcosine + $02 = glycine + serine + HCHO. The cofactor requirements of this reaction have not been established, although Mackenzie (1) has mentioned unpublished results which seemed to indicate that a folic acid deficiency in r...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2005
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi051898d